OsUEV1B, an Ubc enzyme variant protein, is required for phosphate homeostasis in rice

Phosphorus is a crucial macronutrient for plant growth and development. The mechanisms maintaining inorganic phosphate (Pi) homeostasis in rice are not well understood. ubiquitin-conjugating enzyme variant protein OsUEV1B was previously found to interact with OsUbc13 mediate lysine63-linked polyubiquitination. In the present study, we specifically inhibited by Pi deficiency, localized nucleus cytoplasm. Both osuev1b mutant OsUEV1B-RNA interference (RNAi) lines displayed serious symptoms of toxicity due overaccumulation. Some starvation inducible transporter genes were upregulated OsUEV1B-RNAi plants association enhanced acquisition, representative responses, including stimulation acid phosphatase activity root hair growth, also activated presence sufficient Pi. A yeast two-hybrid screen revealed an interaction between OsVDAC1, which confirmed bimolecular fluorescence complementation firefly split-luciferase assays. OsVDAC1 encoded voltage-dependent anion channel mitochondria, shown via Under conditions, similar osuev1b, mutation resulted significantly greater concentrations roots second leaves, improved activity, expression compared wild-type DongJin, whereas overexpression had opposite effects. or knockout reduced mitochondrial membrane potential adenosine triphosphate levels. Moreover, partially restored its high concentration level those DongJin. Our results indicate that required homeostasis.